The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis.
Cell invasion assays have become a critical technique in screening compounds that can inhibit metastasis or promote angiogenesis.
Technology OverviewCellular Invasion is done using Oris kits. In this assay a rubber cell stopper is used which can be fixed on the plate. This stopper does not allow the cells to adhere in the center of the well (Figure 1). This inhibition of the cell growth acts as a detection zone. The cells are overlaid will Collagen Type I and incubated. Cells invade and cover the detection zone. After a fixed time point the cells are stained with a fluorescent dye and read on a plate reader.
Figure 1. HT-1080 Cell Invasion Microscopic Data Ref. Oris Technologies
Service DesignClients can submit plant extracts, synthetic compounds, peptides, formulations etc for invasion testing using MTT assay. Invasion can be tested on various human normal and cancer cell lines. More details about the cell lines can be obtained from DL cell line directory or by contacting us. We provide customized assay design. Customers can select the parameters like
- Drug concentration to be tested
- Time of incubation of the drug with cells
- Duplication of well
- Internal standards
- Cell line